MetabolicMarkers

Educational content only, not medical advice. We are not a medical provider; review your results with a licensed physician. Details

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Hypothyroidism & Hashimoto's Blood Tests: TSH, Free T4 & Antibodies

Thyroid hormone and antibody testing in one place, from a single TSH check to comprehensive 17-test panels.

Affiliate disclosure: we earn a commission when you purchase lab tests through links on this site, at no extra cost to you. Learn more

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Individual thyroid tests from $17.56 and 3-test panels from $55.96 with the 20% promotion applied (pricing at time of writing).

At a glance

Hypothyroidism and Hashimoto's testing measures thyroid hormones, TSH, Free T4, and Free T3, along with thyroid peroxidase (TPO) and thyroglobulin antibodies, the markers associated with autoimmune thyroid activity. These panels are designed for adults experiencing persistent fatigue, unexplained weight gain, cold sensitivity, or hair thinning, and for anyone tracking existing thyroid results over time. Options range from a single TSH test to 17-test panels that add metabolic and nutritional markers.

Best suited for

  • Adults with symptoms such as persistent fatigue, unexplained weight gain, cold sensitivity, dry skin, or thinning hair who want objective thyroid data to review with their doctor
  • People with a family history of thyroid or autoimmune conditions who want a baseline that includes TPO and thyroglobulin antibody markers
  • Anyone on thyroid medication who wants to track TSH, Free T4, and Free T3 between appointments
  • Women noticing heavy or irregular menstrual cycles, mood changes, or memory issues that may warrant a thyroid check
  • Health-conscious adults who want to monitor thyroid trends alongside metabolic and nutritional markers

What's included

TH-1 Hypothyroidism Panel

Free T3, Free T4, and TSH (3 tests, 3 biomarkers)

TH-2 Hypothyroidism Panel

13 tests, 76 biomarkers, adds metabolic and nutritional markers

TH-3 Hypothyroidism Panel

15 tests, 79 biomarkers, adds autoimmune markers

TH-4 Hypothyroidism Panel

17 tests, 81 biomarkers, the most comprehensive option

Thyroid Foundation Panel

13 tests, 23 biomarkers

Thyroid Foundation Panel Plus Thyroid Panel Add-On

15 tests, 78 biomarkers

TH-1 Hashimoto's Disease Panel

Free T3, Free T4, and TSH (3 tests, 3 biomarkers)

TH-2 Hashimoto's Disease Panel

13 tests, 76 biomarkers

TH-3 Hashimoto's Disease Panel

15 tests, 79 biomarkers

TH-4 Hashimoto's Disease Panel

17 tests, 81 biomarkers

TSH Test

T4 Free Test

T3 Free Test

T4 Total Test

T3 Total Test

T3 Reverse (rT3) Test

Thyroid Peroxidase and Thyroglobulin Antibodies Test

TPO and TgAb autoimmune markers

Deep-dive the key markers: TSH, Free T3, Free T4

Key biomarker ranges

Commonly used adult reference ranges for markers this panel measures, labs vary, so always interpret results against your lab's stated ranges and with your doctor.

TSH (µIU/mL)

Commonly used reference ranges run roughly 0.4–4.0 µIU/mL; TSH moves inversely to thyroid activity, and time of day, illness, and biotin can skew results.

TSH reference ranges (µIU/mL)
ZoneRange
Low00.4 µIU/mL
Normal range0.44 µIU/mL
Mildly elevated46 µIU/mL
High610 µIU/mL
Free T3 (pg/mL)

Commonly used reference ranges run roughly 2.3–4.2 pg/mL; Free T3 is best interpreted alongside TSH and Free T4 rather than in isolation.

Free T3 reference ranges (pg/mL)
ZoneRange
Low02.3 pg/mL
Normal range2.34.2 pg/mL
High4.26 pg/mL
Free T4 (ng/dL)

Commonly used reference ranges run roughly 0.8–1.8 ng/dL; Free T4 shows thyroid hormone supply, while TSH shows the pituitary’s demand signal.

Free T4 reference ranges (ng/dL)
ZoneRange
Low00.8 ng/dL
Normal range0.81.8 ng/dL
High1.83 ng/dL

The importance of testing

The thyroid helps regulate metabolism, energy production, and hormonal balance, so even modest shifts in thyroid hormone output can show up as fatigue, weight change, cold sensitivity, dry skin and thinning hair, constipation, or mood and memory changes. Symptoms alone cannot distinguish thyroid patterns from other causes, blood testing shows what your thyroid is actually doing.

A core thyroid workup measures three values. TSH reflects the pituitary's signal telling the thyroid how hard to work, while Free T4 and Free T3 measure the active, unbound hormone circulating in your blood. Read together, these values reveal patterns associated with an underactive thyroid and establish a baseline you can track over time and review with your doctor.

Antibody testing adds another layer. Thyroid peroxidase (TPO) and thyroglobulin antibodies are markers associated with Hashimoto's thyroiditis, the autoimmune process that is the most common cause of an underactive thyroid. Because autoimmune thyroid changes often progress quietly, antibody results can add useful context to borderline hormone values, something worth discussing with your healthcare provider.

The larger TH-2 through TH-4 panels extend beyond thyroid hormones to broader metabolic and nutritional markers, offering a wider picture of how thyroid function fits into overall health. If you already take thyroid medication, periodic retesting helps you and your doctor track whether your levels are staying within your target range.

Promo code

THHYPO20

Save 20% with code THHYPO20, current promotion; confirm final pricing on the partner page.

Ready to get your numbers? Order online, visit a nearby draw site, and receive private results to review with your doctor.

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Frequently asked questions

What do hypothyroidism and Hashimoto's blood tests measure?

They measure thyroid hormone levels, TSH, Free T4, and Free T3, and, in the antibody-inclusive panels, thyroid peroxidase (TPO) and thyroglobulin antibodies, which are markers associated with autoimmune thyroid activity. Larger panels such as TH-2 through TH-4 also include metabolic and nutritional markers for a broader picture. Results give you and your doctor objective data on thyroid function to review together.

Which thyroid panel should I choose?

The TH-1 panel (Free T3, Free T4, and TSH) is the most popular starting point for a first look at thyroid function. If you want antibody markers associated with Hashimoto's thyroiditis, choose a panel that includes TPO and thyroglobulin antibodies, such as the TH-3 or TH-4 options. The most comprehensive TH-4 panels add metabolic and nutritional markers across 17 tests and 81 biomarkers; your doctor can help you decide how much depth is useful for your situation.

What is the difference between TSH, Free T4, and Free T3?

TSH is the pituitary hormone that signals the thyroid to produce hormone, while Free T4 and Free T3 measure the active, unbound thyroid hormones circulating in your blood. TSH often shifts before hormone levels do, which is why it is a common first screen, and Free T4 and Free T3 show how much usable hormone is actually available to your tissues. Reviewing all three together with your doctor gives a fuller view than any single value.

Can I order these thyroid tests without a doctor's visit?

Yes, the panels on this promotion are ordered directly online through the partner page, so no prior doctor's appointment is needed to purchase testing. After ordering, you complete a blood draw at a local lab and receive your results. Results are for informational purposes and should be reviewed with your own healthcare provider, especially before starting or changing any therapy.

Is this thyroid testing available in every US state?

No, direct-to-consumer lab testing is restricted or prohibited in New York, New Jersey, and Rhode Island, so residents of those states may be blocked at the partner's checkout. Availability is broad across most other US states, and the checkout process will confirm whether service is offered in your area.

How often should thyroid levels be retested?

There is no single schedule that fits everyone, retesting frequency depends on your results, symptoms, and whether you take thyroid medication, so follow your doctor's guidance. Many people tracking borderline values or medication adjustments retest periodically to see whether TSH, Free T4, and Free T3 are trending within their target range. Consistent testing over time makes trends easier for you and your doctor to interpret.